BPC-157 reconstitution calculator
Calculate BPC-157 dosing by body weight using published Sikiric et al. protocols. Includes reconstitution math, syringe units, injury-type adjustments, and TB-500 stack guidance.
BPC-157 (Body Protection Compound 157) is a pentadecapeptide — a 15 amino acid sequence — derived from a protective protein found in human gastric juice. It has been the subject of over 100 published papers, primarily from the laboratory of Predrag Sikiric at the University of Zagreb, examining its effects on tissue repair across multiple organ systems.
The compound demonstrates cytoprotective and healing effects through multiple mechanisms: upregulation of growth hormone receptors in tendon fibroblasts, acceleration of collagen synthesis, promotion of angiogenesis (new blood vessel formation), modulation of the nitric oxide system, and direct effects on dopamine and serotonin neurotransmission. In animal models, it has consistently accelerated healing of tendons, muscles, bones, and gastrointestinal tissue.
This calculator implements weight-based dosing protocols derived from Sikiric et al. research. The standard research range of 2–4 mcg/kg/day reflects the doses showing efficacy in published animal studies. Human extrapolation from animal data is imprecise, but this range is widely used as the basis for research protocols.
Injury type selection adjusts the dose multiplier based on condition-specific protocols: tendon and ligament injuries use a higher dose multiplier with local injection guidance; gastrointestinal conditions use a lower systemic dose with oral administration as an alternative. Recovery phase selection determines the dosing intensity and cycle length appropriate to the stage of healing.
This tool also calculates the reconstitution math — concentration, injection volume, and exact syringe units — for a U-100 insulin syringe. For research use only.
calc.dailyDose
392 mcg
4.8 mcg/kg
calc.perInjection
196 mcg
2× daily
calc.injectionVolume
0.078 mL
7.8 units (U-100)
calc.recommendedCycle
4 weeks
systemic
calc.timingProtocol
AM and PM injections, inject proximal to injury site for local protocol
calc.stackRecommendation
Stack with TB-500 (2.5mg 2× weekly) for synergistic tendon repair via complementary actin upregulation pathway
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Frequently asked questions
What bacteriostatic water should I use to reconstitute BPC-157?+
Use pharmaceutical-grade bacteriostatic water (0.9% benzyl alcohol in sterile water). Do not use sterile water without benzyl alcohol — it has no preservative and will allow bacterial growth within days. Do not use normal saline. Bacteriostatic water is the universal standard for peptide reconstitution and extends shelf life of the reconstituted solution to approximately 28 days when refrigerated.
How many mcg of BPC-157 per kg of body weight is standard protocol?+
The standard research range derived from Sikiric et al. protocols is 2–4 mcg/kg/day. For acute injuries, 4 mcg/kg/day split into two injections is commonly used. For subacute and maintenance phases, 1.5–2.5 mcg/kg/day is typical. A 80kg individual would use approximately 160–320 mcg per day depending on phase and injury type.
Should I inject BPC-157 near the injury or inject systemically?+
Both approaches are used. Systemic subcutaneous injection (typically abdomen or thigh) produces measurable plasma levels that act on multiple sites. Local injection — subcutaneously proximal to the injury site — provides higher local concentration at the target tissue. Most protocols for tendon and muscle injuries use local injection with twice-daily dosing. For gut healing, oral administration (dissolved in water or as a capsule) may provide superior mucosal exposure.
Can I stack BPC-157 with TB-500?+
Yes — BPC-157 and TB-500 are frequently combined for synergistic tissue repair. They operate via complementary mechanisms: BPC-157 works primarily through growth hormone receptor upregulation and nitric oxide signaling, while TB-500 (Thymosin Beta-4 fragment) acts through actin upregulation and cell migration. Together they address multiple pathways in the healing cascade. A common protocol is BPC-157 (twice daily) + TB-500 (2–5mg twice weekly).
What is the half-life of BPC-157?+
BPC-157 has a short half-life of approximately 4 hours via subcutaneous injection. This half-life is why twice-daily dosing is standard — single daily dosing leaves significant periods without active compound. The short half-life also means BPC-157 must be reconstituted and stored properly; it does not accumulate with repeated dosing.
Research background
toolShell.references
Sikiric P, Seiwerth S, Rucman R, et al. (2018). Stable gastric pentadecapeptide BPC 157: novel therapy in gastrointestinal tract title: . Current Pharmaceutical Design. DOI: 10.2174/1381612824666180608101120
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